Journal: Scientific Data

Article Title: Chromatin conformation and histone modification profiling across human kidney anatomic regions

doi: 10.1038/s41597-024-03648-8

Figure Lengend Snippet: Index sequences of 18 CUT&RUN libraries presented in this study.

Article Snippet: For antibody binding, 0.5 µg H3K4me3 (Cat # 13-0041k, EpiCypher) and H3K27me3 (Cat # 13-0055, EpiCypher) antibodies were used and 0.5 µg rabbit IgG antibody (Cat # 13-0042k, EpiCypher) was used as a negative control.

Techniques: Modification

Journal: Scientific Data

Article Title: Chromatin conformation and histone modification profiling across human kidney anatomic regions

doi: 10.1038/s41597-024-03648-8

Figure Lengend Snippet: Sequencing depths of CUT&RUN libraries presented in this work.

Article Snippet: For antibody binding, 0.5 µg H3K4me3 (Cat # 13-0041k, EpiCypher) and H3K27me3 (Cat # 13-0055, EpiCypher) antibodies were used and 0.5 µg rabbit IgG antibody (Cat # 13-0042k, EpiCypher) was used as a negative control.

Techniques: Sequencing, Modification

Journal: Scientific Data

Article Title: Chromatin conformation and histone modification profiling across human kidney anatomic regions

doi: 10.1038/s41597-024-03648-8

Figure Lengend Snippet: Visualization of CUT&RUN peaks. ( a , b ) Circos plots for genome-wide H3K4me3 ( a ) and H3K27me3 ( b ) peak profiles across kidney cortex (outer layer), medulla (middle layer) and papilla (inner layer). Sizes of each chromosome are shown. A region of interest on chromosome 19 is highlighted with arrows and is shown with zoom-in ( c ). ( d , e ) Coverage plots of the called peaks over chromosome 3 ( d ) and chromosome 4 ( e ) for each CUT&RUN library (with duplicates merged).

Article Snippet: For antibody binding, 0.5 µg H3K4me3 (Cat # 13-0041k, EpiCypher) and H3K27me3 (Cat # 13-0055, EpiCypher) antibodies were used and 0.5 µg rabbit IgG antibody (Cat # 13-0042k, EpiCypher) was used as a negative control.

Techniques: Genome Wide

Journal: Cell reports

Article Title: SMYD5 is a regulator of the mild hypothermia response

doi: 10.1016/j.celrep.2024.114554

Figure Lengend Snippet:

Article Snippet: The following antibodies were used for the CUT&RUN: H3K36me3 (Thermo Fisher Scientific, MA5–24687), H4K20me3 (EpiCypher, 13–0054), H3K4me3 (EpiCypher, 13–0041 or Abcam, ab8580), H3K27me3 (Thermo Fisher Scientific, MA5–11198), H3K9me3 (Abcam, ab176916), rabbit anti-SMYD5, and Rabbit IgG Antibody (Epicypher, 23613–0042).

Techniques: Mutagenesis, Inhibition, Reverse Transcription, BIA-KA, Sample Prep, CRISPR, Recombinant, Plasmid Preparation, Software